Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

0:42

Tissue Lysate Preparation

1:51

Quantification and Quality Check of Tissue Lysates

2:33

Enzymatic Digestion of Proteins

3:57

Desalting of Digested Peptides

5:16

Quantification of Desalted Peptides

5:58

Labeling of Digested Peptides using iTRAQ Reagents

8:14

Liquid Chromatography Set-up

9:25

Mass Spectrometry Set-up

12:30

Representative Results

13:45

Conclusions