Results: Alternative Splicing and Polyadenylation in RNA-Seq Data
7:46
Conclusion
Transkript
This protocol provides a comprehensive understanding of gene isoforms generated by alternative splicing and polyadenylation by providing a step-by-step workflow to identify differential splicing sites, differentially-expressed exons, and poly(A)si
Sign in or start your free trial to access this content
Alternative splicing (AS) and alternative polyadenylation (APA) expand the diversity of transcript isoforms and their products. Here, we describe bioinformatic protocols to analyze bulk RNA-seq and 3' end sequencing assays to detect and visualize AS and APA varying across experimental conditions.