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Free-floating Immunostaining of Mouse Brains

11.3K Views

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07:58 min

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October 7th, 2021

DOI :

10.3791/62876-v

October 7th, 2021

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11,336 Views

Longlong Tu¹, Nan Zhang^1,2,3, Kristine M Conde¹, Jonathan C Bean¹, Chunmei Wang¹, Yong Xu^1,4

¹USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, ²Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ³Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorder, ⁴Department of Molecular and Cellular Biology, Baylor College of Medicine

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Keywords Immunostaining

Neuroscience Research

Transcardial Perfusion

Formalin Fixation

Brain Isolation

Sliding Microtome

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