Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

0:58

Ambient, Vacuum, and Heat-Mediated Drying of Clostridioides difficile and Enterococcus faecalis Bacterial Macrocolonies

2:33

MALDI Matrix Application via Robotic Spraying

3:12

Preparing Bacterial Macrocolonies for MALDI Imaging Mass Spectrometry Data Acquisition

4:37

Imaging Mass Spectrometry Data Analysis and Compound Identification

5:57

Cryosectioning of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues

7:45

Preparation of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues for Matrix Application and MALDI Imaging Mass Spectrometry

8:08

Results: Imaging Mass Spectrometry Analysis of Bacterial Macrocolonies and Infected Murine Cecal Tissues

9:09

Conclusion