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Tokyo Metropolitan University

3 ARTICLES PUBLISHED IN JoVE

Biology

Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus

Ke Ren¹, Yidan Dai¹, Kai Yi², Masanobu Kinoshita¹, Masahiro Itoh², Ichiro Sakata³, Takafumi Sakai³, Shuang-Qin Yi¹

¹Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, ²Department of Anatomy, Tokyo Medical University, ³Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University

A whole-mount immunohistochemical approach, to visualize neurofilament protein expression in the extrahepatic biliary tract in Suncus murinus. is presented here. This protocol can be used to analyze the innervation of all visceral organs in S. murinus or other species.

Research

Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere

Naoto Kakuta¹, Keisuke Nishijima¹, Van Cuong Han¹, Yuki Arakawa¹, Katsuya Kondo², Yukio Yamada³

¹Department of Mechanical Engineering, Tokyo Metropolitan University, ²Department of Electrical and Electronic Engineering, Tottori University, ³Brain Science Inspired Life Support Research Center, The University of Electro-Communications

A technique utilizing wavelengths of 1150 and 1412 nm to measure the temperature of water surrounding an induction-heated small magnetic sphere is presented.

Environment

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on ¹⁴NH₄⁺/¹⁵NH₄⁺ Analyses via Sequential Conversion to N₂O

Megumi Kuroiwa¹, Keitaro Fukushima^2,3, Kazuma Hashimoto², Yukiko Senga⁴, Tsubasa Sato⁴, Chie Katsuyama⁵, Yuichi Suwa¹

¹Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, ²Faculty & Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, ³Center for Ecological Research, Kyoto University, ⁴Department of Chemistry, Faculty of Science, Toho University, ⁵Graduate School of Integrated Arts and Sciences, Hiroshima University

A series of methods to determine the potential DNRA rate based on ¹⁴NH₄⁺/¹⁵NH₄⁺ analyses is provided in detail. NH₄⁺ is converted into N₂O via several steps and analyzed using quadrupole gas chromatography–mass spectrometry.