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Belgian Red Cross-Flanders

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Britt Van Aelst¹, Hendrik B. Feys¹, Rosalie Devloo¹, Philippe Vandekerckhove^2,3,4, Veerle Compernolle^1,2,4

¹Transfusion Research Center, Belgium Red Cross-Flanders, ²Blood Service, Belgium Red Cross-Flanders, ³Department of Public Health and Primary Care, Catholic University of Leuven, ⁴Faculty of Medicine and Health Sciences, University of Ghent

Platelet transfusion and hemostasis was modeled using blood reconstitution and microfluidic flow chambers to investigate the function of blood banking platelets. The data demonstrate the consequences of platelet storage lesion on hemostasis, in vitro.

Bioengineering

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Katrijn R. Six^1,2, Rosalie Devloo¹, Britt Van Aelst¹, Philippe Vandekerckhove^2,3,4, Hendrik B. Feys¹, Veerle Compernolle^1,2,3

¹Transfusion Research Center, Belgian Red Cross-Flanders, ²Faculty of Medicine and Health Sciences, Ghent University, ³Blood Service, Belgian Red Cross-Flanders, ⁴Department of Public Health and Primary Care, KULeuven - University of Leuven

This paper describes an experimental model of hemostasis that simultaneously measures platelet function and coagulation. Platelet and fibrin fluorescence is measured in real-time, and platelet adhesion rate, coagulation rate, and onset of coagulation are determined. The model is used to determine platelet procoagulant properties under flow in concentrates for transfusion.