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Queensland University of Technology (QUT)

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Melt Electrospinning Writing of Three-dimensional Poly(ε-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications

Felix M. Wunner¹, Onur Bas¹, Navid T. Saidy¹, Paul D. Dalton², Elena M. De-Juan Pardo¹, Dietmar W. Hutmacher^1,3,4

¹ARC ITTC in Additive Biomanufacturing, Institute for Health and Biomedical Innovation (IHBI), Queensland University of Technology (QUT), ²Department for Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University of Würzburg, ³Institute for Advanced Study, Technical University of Munich (TUM), ⁴George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology

This protocol serves as a comprehensive guideline to fabricate scaffolds via electrospinning with polymer melts in a direct writing mode. We systematically outline the process and define the appropriate parameter settings for achieving targeted scaffold architectures.

Bioengineering

Design of an Open-Source, Low-Cost Bioink and Food Melt Extrusion 3D Printer

Matthew Lanaro^*1, Jacob Skewes^*1, Logan Spiers¹, Prasad K. Yarlagadda¹, Maria A. Woodruff¹

¹Science and Engineering Faculty, Queensland University of Technology (QUT)

The aim of this work is to design and construct a reservoir-based melt extrusion three-dimensional printer made from open-source and low-cost components for applications in the biomedical and food printing industries.

Bioengineering

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

Sebastian Eggert^1,2, Melanie Kahl^1,3, Ross Kent¹, Lukas Gaats¹, Nathalie Bock^1,3,4,5, Christoph Meinert^1,2, Dietmar W. Hutmacher^1,2,3,4,6

¹Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology, ²School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty, Queensland University of Technology, ³School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, ⁴Australian Prostate Cancer Research Centre, Institute of Health and Biomedical Innovation, Queensland University of Technology, ⁵Translational Research Institute, Queensland University of Technology, ⁶ARC ITTC in Additive Biomanufacturing, Institute of Health and Biomedical Innovation, Queensland University of Technology

This protocol serves as a comprehensive tutorial for standardized and reproducible mixing of viscous materials with a novel open source automation technology. Detailed instructions are provided on the operation of a newly developed open source workstation, the usage of an open source protocol designer, and the validation and verification to identify reproducible mixtures.