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European Molecular Biology Laboratory, Hamburg Outstation c/o DESY

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Fixed Target Serial Data Collection at Diamond Light Source

Sam Horrell¹, Danny Axford¹, Nicholas E. Devenish¹, Ali Ebrahim¹, Michael A. Hough², Darren A. Sherrell^1,3, Selina L. S. Storm^1,4, Ivo Tews⁵, Jonathan A. R. Worrall², Robin L. Owen¹

¹Diamond Light Source, Harwell Science and Innovation Campus, ²School of Life Sciences, University of Essex, ³X-ray Science Division, Argonne National Laboratory, ⁴European Molecular Biology Laboratory, Hamburg Outstation c/o DESY, ⁵Biological Sciences, Institute for Life Sciences, University of Southampton

We present a comprehensive guide to fixed target sample preparation, data collection, and data processing for serial synchrotron crystallography at Diamond beamline I24.