A Method for Generating Pulmonary Neutrophilia Using Aerosolized LipopolysaccharideAbraham B. Roos 1, Tove Berg 1, Kerstin M. Ahlgren 1, Johan Grunewald 1, Magnus Nord 1,2
1Department of Medicine, Solna and CMM, Respiratory Medicine Unit, Karolinska Institutet, 2Safety Science, Global Regulator Affairs & Patient Safety, AstraZeneca Global Medicines Development
We describe a method for inducing neutrophilic pulmonary inflammation by challenge to aerosolized lipopolysaccharide by nebulization, to model acute lung injury. In addition, basic surgical techniques for lung isolation, tracheal intubation and bronchoalveolar lavage are also described.
