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Chonbuk National University

5 ARTICLES PUBLISHED IN JoVE

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Engineering

Visible-light Induced Reduction of Graphene Oxide Using Plasmonic Nanoparticle
Dinesh Kumar 1, Ah-Reum Lee 1, Sandeep Kaur 2, Dong-Kwon Lim 1
1KU-KIST Graduate School of Converging Science and Technology, Korea University, 2Department of BIN Fusion Technology, Chonbuk National University

A simple protocol for the preparation of reduced graphene oxide using visible light and plasmonic nanoparticle is described.

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Engineering

Experimental Implementation of a New Composite Fabrication Method: Exposing Bare Fibers on the Composite Surface by the Soft Layer Method
Dongyoung Lee 1, Dai Gil Lee 1, Jun Woo Lim 2
1Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 2LANL-CBNU Engineering Institute Korea, Chonbuk National University

A protocol to expose bare fibers on the composite surface by eliminating resin rich area is presented. The fibers are exposed during fabrication of the composites, not by the post surface treatment. The exposed carbon composites exhibit high electrical conductivity in the through-thickness direction and high mechanical property.

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Neuroscience

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
Sangyun Jeong 1
1Department of Molecular Biology, Chonbuk National University

This work details a standard immunohistochemistry method to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. The filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition during neural development.

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Genetics

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
Ju-Chun Chang 1, Se Jin Lee 2, Jae Su Kim 2, Chung-Hsiung Wang 3, Yu-Shin Nai 1
1Department of Biotechnology and Animal Science, National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, 3Department of Entomology, National Taiwan University

This protocol describes a heat shock-induced protein expression system (pDHsp/V5-His/sf9 cell system), which can be used for either expressing foreign proteins or evaluating the anti-apoptotic activity of potential foreign proteins and their truncated amino acids in insect cells.

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Biology

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests
Yao-Chia Liu 1, Nian-Tong Ni 1, Ju-Chun Chang 1, Yi-Hsuan Li 1, Mi Rong Lee 2, Jae Su Kim 2,3, Yu-Shin Nai 1
1Department of Entomology, National Chung Hsing University, 2Department of Agricultural biology, Jeonbuk National University, 3Department of Agricultural Convergence Technology, Jeonbuk National University

Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field.

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