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Haukeland University Hospital

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives
Kristina Xiao Liang *1,2, Anbin Chen *1,2,3,4, Cecilie Katrin Kristiansen 1,2, Laurence A. Bindoff 1,2
1Department of Clinical Medicine (K1), University of Bergen, 2Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, 3Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine, Shandong University, 4Shandong Key Laboratory of Brain Function Remodeling

This study reports a novel approach to measure multiple mitochondrial functional parameters based on flow cytometry and double staining with two fluorescent reporters or antibodies to detect changes in mitochondrial volume, mitochondrial membrane potential, reactive oxygen species level, mitochondrial respiratory chain composition, and mitochondrial DNA.

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Neuroscience

Application of Flow Cytometric Analysis for Measuring Multiple Mitochondrial Parameters in 3D Brain Organoids
Sharika Marjan 1,2, Tsering Yangzom 1,2, Cecilie Katrin Kristiansen 1,3, Anbin Chen 4, Laurence A. Bindoff 1,5,6, Kristina Xiao Liang 1,3
1Department of Clinical Medicine (K1), University of Bergen, 2Centre for International Health, Department of Global Public Health and Primary Care, University of Bergen, 3Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, 4Department of Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 5Department of Neurology, Haukeland University Hospital, 6National Advisory Unit for Congenital Metabolic Diseases, Oslo University Hospital

This protocol reports a unique method of using a streaming cytometer and multiple antibodies for simultaneous assessment of multiple mitochondrial functional parameters, including changes in mitochondrial volume, amounts of the mitochondrial respiratory chain (MRC) complex subunits, and mitochondrial DNA (mtDNA) replication.

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