Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term InfantsSota Iwatani 1, Makiko Yoshida 2, Keiji Yamana 1, Daisuke Kurokawa 1, Jumpei Kuroda 1, Khin Kyae Mon Thwin 1, Suguru Uemura 1, Satoru Takafuji 1, Nanako Nino 1, Tsubasa Koda, Masami Mizobuchi 4, Masahiro Nishiyama 1, Kazumichi Fujioka 1, Hiroaki Nagase 1, Ichiro Morioka 5, Kazumoto Iijima 1, Noriyuki Nishimura 1
1Department of Pediatrics, Kobe University Graduate School of Medicine, 2Department of Pathology, Kobe Children's Hospital, 3Department of Pediatrics, Hyogo College of Medicine, 4Department of Developmental Pediatrics, Shizuoka Children's Hospital, 5Department of Pediatrics, Nihon University School of Medicine
Human umbilical cord (UC) can be obtained during the perinatal period as a result of preterm, term, and postterm delivery. In this protocol, we describe the isolation and characterization of UC-derived mesenchymal stem cells (UC-MSCs) from fetuses/infants at 19-40 weeks of gestation.