Method for Obtaining Primary Ovarian Cancer Cells From Solid SpecimensLee J. Pribyl 1, Kathleen A. Coughlin 1, Thanasak Sueblinvong 1, Kristin Shields 2, Yoshie Iizuka 1,3, Levi S. Downs 1,3, Rahel G. Ghebre 1,3, Martina Bazzaro 1,3
1Department of Obstetrics, Gynecology, and Women's Heath, University of Minnesota, 2Department of Obstetrics and Gynecology, Maricopa Medical Center and St Josephs Hospital and Medical Center, 3Masonic Cancer Center, University of Minnesota
This study describes a detailed method for isolation and characterization of primary ovarian cancer cells from solid clinical specimens. Ovarian cancer clinical specimens are subjected to enzymatic digestion to obtain viable, fibroblast-free epithelial ovarian cancer (EOC) cells highly suitable for downstream applications.
