NYU School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Medicine

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Stephen T. Armenti¹, Jonathan P. Greenberg², R. Theodore Smith¹

¹Department of Ophthalmology, NYU School of Medicine, ²Harkness Eye Institute, Columbia University

The retinal pigment epithelium (RPE) supports the sensory retina through recycling visual cycle byproducts, which accumulate as lipofuscin. These products are autofluorescent and can be qualitatively imaged in vivo. Here, we describe a method to quantitatively image RPE lipofuscin using confocal scanning laser ophthalmoscopy.

Developmental Biology

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

Wenyue Guan¹, Lalanti Venkatasubramanian², Myungin Baek³, Richard S. Mann², Jonathan Enriquez¹

¹Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, ²Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, ³Neuroscience Program, NYU School of Medicine

Here we describe a protocol to visualize the axonal targeting with a florescent protein in adult legs of Drosophila by fixation, mounting, imaging, and post-imaging steps.