Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous ImmunityOdaelys Walwyn *1, Sini Skariah *1, Brian Lynch 1, Nathaniel Kim 1, Yukari Ueda 1, Neal Vohora 1, Josh Choe 1, Dana G. Mordue 1
1Department of Microbiology and Immunology, New York Medical College
Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.
