JoVE Logo

Sign In

Niigata University

4 ARTICLES PUBLISHED IN JoVE

image

Biology

Preparation of Giant Vesicles Exhibiting Visible-light-induced Morphological Changes
Masanari Hirahara 1, Akira Tsukamoto 2, Hiroki Goto 1, Shigeru Tada 2, Masayuki Yagi 3, Yasushi Umemura 1
1Department of Applied Chemistry, School of Applied Science, National Defense Academy of Japan, 2Department of Applied Physics, School of Applied Science, National Defense Academy of Japan, 3Department of Materials Science and Technology, Faculty of Engineering, Niigata University

The synthesis of ruthenium complex surfactants exhibiting photoisomerization in giant vesicles is described. The preparation and light irradiation of the giant vesicles are also described.

image

Engineering

In Situ Synthesis of Gold Nanoparticles without Aggregation in the Interlayer Space of Layered Titanate Transparent Films
Kazuhisa Sasaki 1, Kazuki Matsubara 1, Shiori Kawamura 1, Kenji Saito 1, Masayuki Yagi 1, Tatsuto Yui 1
1Department of Material Science and Technology, Faculty of Engineering, Niigata University

Here, we present a protocol for the in situ synthesis of gold nanoparticles (AuNPs) within the interlayer space of layered titanate films without the aggregation of AuNPs. No spectral change was observed even after 4 months. The synthesized material has expected applications in catalysis, photo-catalysis, and the development of cost-effective plasmonic devices.

image

Neuroscience

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light
Atsushi Sugie 1,2,5, Christoph Möhl 3, Satoko Hakeda-Suzuki 4, Hideaki Matsui 1,2, Takashi Suzuki *4, Gaia Tavosanis *5
1Department of Neuroscience of Disease, Center for Transdisciplinary Research, Niigata University, 2Brain Research Institute, Niigata University, 3Image and Data Analysis Facility, German Center for Neurodegenerative Diseases (DZNE), 4Graduate School of Life Science and Technology, Tokyo Institute of Technology (Titech), 5Dendrite Differentiation, German Center for Neurodegenerative Diseases (DZNE)

Here we show how to quantify the number and spatial distribution of synaptic active zones in Drosophila melanogaster photoreceptors, highlighted with a genetically encoded molecular marker, and their modulation after prolonged exposure to light.

image

Biology

Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate
Yen Xuan Ngo 1,2,3, Kenta Haga 4, Ayako Suzuki 4, Hiroko Kato 4, Hiromi Yanagisawa 1,5, Kenji Izumi 4, Aiko Sada 1,3
1Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, 3International Research Center for Medical Sciences (IRCMS), Kumamoto University, 4Division of Biomimetics, Faculty of Dentistry and Graduate School of Medical and Dental Sciences, Niigata University, 5Faculty of Medicine, University of Tsukuba

The present protocol describes the isolation and culture of oral keratinocytes derived from the adult mouse palate. An evaluation method using immunostaining is also reported.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved