Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch DiagnosticsKatariina Jaenes *1, Severino Jefferson Ribeiro da Silva *1,2, Justin R. J. Vigar *1, Kaiyue Wu 3,4, Masoud Norouzi 1, Pouriya Bayat 1, Margot Karlikow 1, Seray Cicek 1, Yuxiu Guo 1, Alexander A. Green 3,4, Lindomar Pena 2, Keith Pardee 1,5
1Leslie Dan Faculty of Pharmacy, University of Toronto, 2Laboratory of Virology and Experimental Therapy (LAVITE), Department of Virology, Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (Fiocruz), 3Department of Biomedical Engineering, Boston University, 4Molecular Biology, Cell Biology & Biochemistry Program, Graduate School of Arts and Sciences, Boston University, 5Department of Mechanical and Industrial Engineering, University of Toronto
Access to decentralized, low-cost, and high-capacity diagnostics that can be deployed into the community for decentralized testing is critical for combating global health crises. This manuscript describes how to build paper-based diagnostics for viral RNA sequences that can be detected with a portable optical reader.
