Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondiiLeah M. Rommereim 1, Miryam A. Hortua Triana 1, Alejandra Falla 1, Kiah L. Sanders 1, Rebekah B. Guevara 1, David J. Bzik 1, Barbara A. Fox 1
1Department of Microbiology and Immunology, The Geisel School of Medicine at Dartmouth
Here we report a method for using type I and type II Δku80 strains of Toxoplasma gondii to efficiently generate targeted gene deletions and gene replacements for functional genomic analysis.
