Enrichment of Mammalian Tissues and Xenopus Oocytes with CholesterolAlexandria Slayden 1, Kelsey North 1, Shivantika Bisen 1, Alex M. Dopico 1, Anna N. Bukiya 1, Avia Rosenhouse-Dantsker 2
1Department of Pharmacology, Addiction Science and Toxicology, The University of Tennessee HSC, 2Department of Chemistry, University of Illinois at Chicago
Two methods of cholesterol enrichment are presented: the application of cyclodextrin saturated with cholesterol to enrich mammalian tissues and cells, and the use of cholesterol-enriched phospholipid-based dispersions (liposomes) to enrich Xenopus oocytes. These methods are instrumental for determining the impact of elevated cholesterol levels in molecular, cellular, and organ function.
