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The University of Western Australia

5 ARTICLES PUBLISHED IN JoVE

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Biology

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro
Marcus K. Giacci 1, Nathan S. Hart 2, Richard V. Hartz 1, Alan R. Harvey 3, Stuart I. Hodgetts 3, Melinda Fitzgerald 1
1Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, 2School of Animal Biology and The Oceans Institute, The University of Western Australia, 3Experimental and Regenerative Neurosciences, School of Anatomy, Physiology and Human Biology, The University of Western Australia

Non-coherent Xenon light was passed through narrow-band interference and neutral density filters to deliver light of varying wavelength and intensity to cultured cells. This protocol was used to assess the effects of red/near-infrared light therapy on production of reactive species in vitro: no effects were observed using the tested parameters.

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Immunology and Infection

A Modified Murine Heterotopic Heart Transplant Protocol Matching Contemporary Standards of Aseptic Technique, Anesthesia, and Analgesia
David A. Singer 1, Gabrielle C. Musk 2, Wen Hua Huang 1,3, Liu Liu 1, Jaskirat Kaur 1, Monalyssa Watson 1, Amy Prosser 1, Michaela Lucas 1,4, Andrew Lucas 5
1Medical School, The University of Western Australia, 2Animal Care Services, The University of Western Australia, 3Western Australian Liver and Kidney Transplant Service, Sir Charles Gairdner Hospital, 4Department of Immunology, Sir Charles Gairdner Hospital and Pathwest Laboratory Medicine, 5School of Biomedical Sciences, The University of Western Australia

The present paper describes a modified technique for heterotopic vascularized cardiac transplantation with updated aseptic technique, analgesia, and anesthesia.

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Biology

Fluorescence Lifetime Macro Imager for Biomedical Applications
Rajannya Sen 1, Alexander V. Zhdanov 1, Ciaran Devoy 2, Mark Tangney 2, Liisa M. Hirvonen 3, Andrei Nomerotski 4, Dmitri B. Papkovsky 1
1School of Biochemistry and Cell Biology, University College Cork, 2Cancer Research@UCC, University College Cork, 3Centre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, 4Physics Department, Brookhaven National Laboratory

This paper describes the use of a new, fast optical imager for the macroscopic photoluminescence lifetime imaging of long decay emitting samples. The integration, image acquisition, and analysis procedures are described, along with the preparation and characterization of the sensor materials for the imaging and the application of the imager in studying biological samples.

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Medicine

Continuous Telemetric In Utero Tracheal Pressure Measurements in Fetal Lambs
Mar Janna Dahl 1, Stefano Robbiani 2, Chiara Veneroni 2, Anna Lavizzari 3, Sebastian Amos 1, Gabrielle C. Musk 1,4, Helen Kershaw 4, Jonathan W. Davis 5,6, Fabio Mosca 3, Peter B. Noble 1, Raffaele Dellacà *2, J. Jane Pillow *1,6
1School of Human Sciences, The University of Western Australia, 2Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano University, 3NICU, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 4Animal Care Services, The University of Western Australia, 5Medical School, University of Western Australia, 6Telethon Kids Institute

Optimization of occlusion pressure and duration required to improve lung hypoplasia with tracheal occlusion is essential to improve in utero corrective treatments for congenital diaphragmatic hernia (CDH). This study reports a new method for continuous measurement of the tracheal pressure in an occluded and unoccluded fetal lamb surgical model of CDH.

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Immunology and Infection

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
Liang Wang *1,2,3, Jin-Xin Lai *1, Yu-Ting Si *1,4, Xu-Xia Cui 1,4, Zeeshan Umar 1,5, Xiao-Jun Ru 1, Xin-Yu Zhang 1, Zheng-Kang Li 1, Alfred Chin Yen Tay 5,6,7,8, Barry J. Marshall 5,6,7,8, Guang-Hua Li 1, Bing Gu 1
1Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, 2Division of Microbiology and Immunology, School of Biomedical Sciences, The University of Western Australia, 3Center for Precision Health, School of Medical and Health Sciences, Edith Cowan University, 4Medical Technology School of Xuzhou Medical University, 5Marshall Laboratory of Biomedical Engineering, School of Medicine, Shenzhen University, 6The Marshall Centre for Infectious Diseases Research and Training, The University of Western Australia, 7Marshall International Digestive Diseases Hospital, Zhengzhou University, 8Marshall Medical Research Center, Fifth Affiliated Hospital of Zhengzhou University

The protocol presents a noninvasive method for the rapid diagnosis of Helicobacter pylori stomach infections through the string test and determines its antibiotic resistance to clarithromycin and levofloxacin using quantitative polymerase chain reaction (qPCR).

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