In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence MicroscopyHtet Ng *1, Masuda Begum *1, Gabriella N. L. Chua *1,2, Shixin Liu 1
1Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, 2Tri-Institutional PhD Program in Chemical Biology
This article presents a detailed experimental procedure for reconstituting nucleosome-containing DNA tethers for single-molecule correlative force and fluorescence microscopy. It further describes several downstream experiments that can be conducted to visualize the binding behavior of chromatin-interacting proteins and analyze changes in the physical properties of nucleosomes.
