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Tsukuba University

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval
Rebecca F. Rosen 1, Yasushi Tomidokoro 2, Jorge A. Ghiso 3, Lary C. Walker 1,4
1Yerkes National Primate Research Center, Emory University, 2Department of Neurology, Institute of Clinical Medicine, Tsukuba University, 3Department of Pathology, New York University School of Medicine, 4Department of Neurology, Emory University

We describe a technique for the preparation of clarified human cortical homogenates, protein separation by SDS-PAGE, antigen retrieval and immunoblotting with an antibody to the Aβ peptide. Using this protocol, we consistently detect monomeric and multimeric Aβ in cortical tissue from humans with Alzheimer's pathology.

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Engineering

Detection and Recovery of Palladium, Gold and Cobalt Metals from the Urban Mine Using Novel Sensors/Adsorbents Designated with Nanoscale Wagon-wheel-shaped Pores
Sherif A. El-Safty 1,2, Mohamed A. Shenashen 1, Masaru Sakai 3, Emad Elshehy 1, Kohmei Halada 1
1National Institute for Materials Science, Japan, 2Graduate School for Advanced Science and Engineering, Waseda University, 3Center for Research in Isotopes and Environmental Dynamics, Tsukuba University

Because of the importance and extensive use of palladium, gold and cobalt metals in high-technology equipment, their recovery and recycling constitute an important industrial challenge. The metal recovery system described herein is a simple, low-cost means for the effective detection, removal, and recovery of these metals from the urban mine.

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