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3 ARTICLES PUBLISHED IN JoVE

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Biology

Laser Capture Microdissection of Mammalian Tissue
Robert A Edwards 1
1Department of Pathology, University of California, Irvine (UCI)

Laser Capture Microdissection of Mammalian Tissue

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Biology

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
Hassan Khalil 1, Wenxian Nie 1, Robert A Edwards 2, James Yoo 1
1Department of Surgery, UCLA, 2Department of Pathology, UC Irvine

The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.

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Bioengineering

Zebra II as A Novel System to Record Electrophysiological Signals in Zebrafish
Ramses Seferino Trigo Torres 1, Mao-Hsiang Huang 2, Mohamed Benomar 2, Tai Le 1, Tim Etchells 3, Xiaolei Xu 4, Michael P. H. Lau 3, Hung Cao 1,2
1Department of Biomedical Engineering, UC Irvine, 2Department of Electrical Engineering and Computer Science, UC Irvine, 3Sensoriis Inc., 4Department of Biochemistry and Molecular Biology, Mayo Clinic

The study introduces Zebra II, an advanced system for prolonged ECG acquisition and analysis in zebrafish. This system features an independent perfusion system and multiple-point electrodes for handling multiple fish in controlled environments. Acute effects of Amiodarone were assessed and analyzed with wild-type zebrafish.

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