Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein LocalizationXavier Morató *1,2, Marc López-Cano *1,2, Paula M. Canas 3, Rodrigo A. Cunha 3, Francisco Ciruela 1,2
1Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL, Universitat de Barcelona, L'Hospitalet de Llobregat, 2Institut de Neurociències, Universitat de Barcelona, 3Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine, University of Coimbra
Here, we present a brain membrane fractionation protocol that represents a robust procedure to isolate proteins belonging to different synaptic compartments.
