University Hospital of Strasbourg

2 ARTICLES PUBLISHED IN JoVE

Medicine

In vivo Macrophage Imaging Using MR Targeted Contrast Agent for Longitudinal Evaluation of Septic Arthritis

Guillaume Bierry^1,2, Sophie Lefevre^2,3, Jean-Louis Dietemann¹, François Jehl^2,3

¹Department of Radiology, University Hospital of Strasbourg, ²EA 3432, University of Strasbourg, ³Department of Bacteriology, University Hospital of Strasbourg

We demonstrate how to perform macrophage MR imaging using ultrasmall superparamagnetic contrast agent (USPIO) in septic arthritis, allowing an initial and longitudinal in vivo non-invasive evaluation of macrophages infiltration and an assessment of therapy efficacy.

Cancer Research

Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions

Quentin Fuchs¹, Aurélie Batut², Mélanie Gleyzes², Jessica Rontard², Louise Miny², Margot Libralato², Janaina Vieira², Delphine Debis², Florian Larramendy², Thibault Honegger², Melissa Messe¹, Marina Pierrevelcin¹, Benoit Lhermitte^1,3, Monique Dontenwill¹, Natacha Entz-Werlé^1,4

¹Team Tumoral signaling and therapeutic targets, UMR CNRS 7021 - Laboratory of Bioimaging and Pathologies, ²NETRI, ³Centre de Ressources Biologiques, Pathology department, University Hospital of Strasbourg, ⁴Pediatric Oncohematology unit, University Hospital of Strasbourg

Recent works uncover the neuronal impact on high-grade pediatric glioma (pHGG) cells and their reciprocal interactions. The present work shows the development of an in vitro model co-culturing pHGG cells and glutamatergic neurons and recorded their electrophysiological interactions to mimic those interactivities.