Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal ImagingIsabel Murillo 1, Mumtaz Virji 1
1Department of Cellular and Molecular Medicine, University of Bristol, UK
Neisseria meningitidis (Nm), a gram negative human-specific respiratory pathogen, can bind to human α-actinin. Here we present a protocol for visualisation of colocalisation of the bacterium with intracellular α-actinin after bacterial entry into human brain microvascular endothelial cells (HBMECs).
