Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish LarvaeFrancois El-Daher 1, Jason J. Early 1, Claire E. Richmond 1, Rory Jamieson 1, Thomas Becker 1,2, Catherina G. Becker 1,2
1Centre for Discovery Brain Sciences, University of Edinburgh Medical School: Biomedical Sciences, 2Center for Regenerative Therapies at the TU Dresden
The present protocol describes a method to induce tissue-specific and highly reproducible injuries in zebrafish larvae using a laser lesion system combined with an automated microfluidic platform for larvae handling.
