Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T CellsMadelyn VanBlunk 1, Pritha Agarwalla 1,2, Sharda Pandit 1,2, Yevgeny Brudno 1,2,3
1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, 2Comparative Medicine Institute, North Carolina State University, 3Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill
Herein is a protocol for creating dry macroporous alginate scaffolds that mediate efficient viral gene transfer for use in genetic engineering of T cells, including T cells for CAR-T cell therapy. The scaffolds were shown to transduce activated primary T cells with >85% transduction.
