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University of Tuebingen

4 ARTICLES PUBLISHED IN JoVE

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Biology

Preparation of Quality Inositol Pyrophosphates
Omar Loss 1, Cristina Azevedo 1, Zsolt Szijgyarto 1, Daniel Bosch 1, Adolfo Saiardi 1
1Medical Research Council (MRC), Cell Biology Unit and Laboratory for Molecular Cell Biology, University College London

Inositol pyrophosphates play an important role in human pathologies such cancer, diabetes and obesity; however, the exact mechanism of action is a matter of dispute. The lack of commercially available inositol pyrophosphates renders detailed studies problematic. Here we describe a simple protocol to produce and isolate milligrams of inositol pyrophosphates.

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Neuroscience

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices
Daniel Bosch 1, Douglas Asede 2, Ingrid Ehrlich 1
1Hertie Institute for Clinical Brain Research and Werner Reichardt Centre for Integrative Neuroscience, University of Tuebingen, 2Max Planck Florida Institute for Neuroscience

Optogenetic approaches are widely used to manipulate neural activity and assess the consequences for brain function. Here, a technique is outlined that upon in vivo expression of the optical activator Channelrhodopsin, allows for ex vivo analysis of synaptic properties of specific long range and local neural connections in fear-related circuits.

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Neuroscience

Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala
Sabine Schönherr 1, Anna Seewald 1, Yu Kasugai 1, Daniel Bosch 2, Ingrid Ehrlich 2, Francesco Ferraguti 1
1Department of Pharmacology, Medical University of Innsbruck, 2Hertie Institute for Clinical Brain Research and Centre for Integrative Neuroscience, University of Tübingen

This article illustrates how the expression of neurotransmitter receptors can be quantified and the pattern analyzed at synapses with identified pre and postsynaptic elements using a combination of viral transduction of optogenetic tools and the freeze-fracture replica immunolabeling technique.

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Environment

Laboratory Simulation of an Iron(II)-rich Precambrian Marine Upwelling System to Explore the Growth of Photosynthetic Bacteria
Markus Maisch 1,2, Wenfang Wu 1, Andreas Kappler 1, Elizabeth D. Swanner 1,2
1Department of Geosciences, University of Tuebingen, 2Department of Geological and Atmospheric Sciences, Iowa State University

We simulated a Precambrian ferruginous marine upwelling system in a lab-scale vertical flow-through column. The goal was to understand how geochemical profiles of O2 and Fe(II) evolve as cyanobacteria produce O2. The results show the establishment of a chemocline due to Fe(II) oxidation by photosynthetically produced O2.

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