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This video introduces chemotaxis in the nematode C. elegans and shows a sample protocol testing chemotactic aversion to copper sulfate.
The following protocol is an excerpt from Campbell et al, A Caenorhabditis elegans Nutritional-Status Based Copper Aversion Assay, J. Vis. Exp. (2017).
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Figure 1: A Visual Representation of Markings to Indicate Copper Solution Placement on the Underside of a 5 cm Petri Dish. These indications are used to ensure that the sections of the plate have been appropriately measured and serve as a guideline when transferring the bacterial patch and then copper solution onto the agar surface.
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Name | Company | Catalog Number | Comments |
M9 Solution [3 g KH2PO4, 6 g Na2HPO4, 5 g NaCl, 1 ml 1 M MgSO4, H2O to 1 litre. Autoclave to sterilize before use.] | Produced in lab | ||
Cupric Sulfate | Sigma | C-1297 | Use water to appropriately suspend to a concentration of 0.5M |
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Source: Campbell, J. C., et al. A Caenorhabditis elegans Nutritional-Status Based Copper Aversion Assay. J. Vis. Exp. (2017).
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