preparation of liujunzi decoction (ljzd) for the treatment of ulcerative coloproctitis-colorectal cancer  (uc-crc)

Decocción de Liujunzi: Un tratamiento eficaz para la prevención del CCU-CRC

Colorectal cancer (CRC) is a common gastrointestinal malignancy, the third most common malignancy, and the second most common cause of death in the world, accounting for 10% of the global cancer incidence and 9.4% of the total cancer-related death1,2. Genetic factors, chronic inflammation, high-fat diet, diabetes, and abnormal intestinal flora are risk factors for CRC3,4. Among them, inflammatory bowel disease, especially ulcerative coloproctitis (UC), is a clear risk factor for CRC5,6. UC-associated CRC (UC-CRC) is a transition process of inflammation, atypical hyperplasia, and cancer based on chronic inflammation of the colorectum, which is different from the typical adenoma-adenocarcinoma development model of CRC7,8. Compared with the general population, the risk of CRC is approximately 10-40 times higher in patients with inflammatory bowel disease9.
Currently, surgery is still the standard treatment for CRC, and depending on the location and stage of the tumor, radiation therapy, systemic drug therapy, or a combination of both are possible10. Although these traditional treatment modalities have made great progress, due to the high heterogeneity and recurrence rate of CRC, the prognosis is poor, and the treatment effect is not ideal11,12. Therefore, early detection, early diagnosis, and comprehensive treatment are key to improving the survival rate of CRC patients, and it is particularly important to pay attention to the transformation of UC to CRC. Over the years, traditional Chinese medicine (TCM) has attracted much attention in the treatment of UC-CRC or chronic gastritis due to its limited side effects and significant efficacy. Based on dialectical treatment, famous Chinese medicine practitioners of various generations have created a large number of classic prescriptions, such as Huangqi Jianzhong decoction13, Sijunzi decoction14, and Sishen pill15.
Liujunzi decoction (LJZD) originated from the works of Yi Xue Zheng Zhuan compiled in the Ming Dynasty and is a classic prescription in TCM16. As shown in Table 1, LJZD consists of six traditional herbs, including Codonopsis pilosula (Franch.) Nannf. (Dangshen), Poria cocos (Schw.) Wolf (Fuling), Atractylodes macrocephala Koidz. (Baizhu), Glycyrrhiza uralensis Fisch. (Gancao), Citrus reticulata Blanco (Chenpi) and Pinellia ternata (Thunb.) Breit (Banxia), which has the effect of replenishing qi and strengthening the spleen, drying dampness, and resolving phlegm. In modern clinical practice, it is often used to treat chronic gastritis, gastric ulcers, and duodenal ulcers. Modern pharmacological research has shown that LJZD and modified LJZD have high application value in the adjuvant treatment of UC and digestive tract cancer17,18,19.
At present, there are many ways to construct UC-CRC mouse models, but the azoxymethane (AOM)/dextran sulfate sodium (DSS) induced mouse model is the most widely used UC-CRC model; the clinical symptoms, morphological, and pathological observations have proved that the model is very similar to human UC-CRC20,21. The basic principle is to first induce carcinogenesis with chemical carcinogen AOM and then continuously expose mice to the inflammatory stimulation environment of DSS to simulate the continuous damage and repair of intestinal mucosal epithelium, thereby constructing a UC-CRC mouse model22. The aim of this study is to establish a mouse model of UC-CRC by intraperitoneal injection of AOM and cyclic stimulation of DSS in the short term and to evaluate the effect of the drug and the molecular mechanism of LJZD on UC-CRC in order to provide a scientific basis for the treatment of UC-CRC.

Autor destacado: La decocción de Liujunzi como tratamiento tradicional chino para el cáncer de coloproctitis

Establecimiento de un modelo de cáncer de coloproctitis en ratones y evaluación del efecto terapéutico de la medicina china

Watch this Scientific Journal Video about Preparation of Liujunzi Decoction LJZD for the Treatment of Ulcerative Coloproctitis-Colorectal Cancer  UC-CRC at JoVE.com

Preparación de la decocción de Liujunzi (LJZD) para el tratamiento de la coloproctitis ulcerosa-cáncer colorrectal (UC-CRC)

Para comenzar, coloque a Dang Shen, Bai Zhu, en una olla de cerámica especializada. Agrega 1000 mililitros de agua destilada y déjala en remojo a temperatura ambiente durante una hora. Con un molinillo, muele 12 gramos de Fu ling hasta obtener un polvo fino.Luego, remoje el Fu ling en polvo en 300 mililitros de agua destilada en un recipiente separado durante una hora a temperatura ambiente. Mezcle la mezcla de hierbas chinas preparada en la olla de cerámica especializada. Hervir la mezcla y mantenerla a fuego medio hasta que el volumen de decocción se reduzca a 300 mililitros.Combine el filtrado obtenido de la decocción y continúe hirviendo hasta que el volumen se reduzca a 150 mililitros. A continuación, centrifugar el líquido concentrado a 10.000 G durante cinco minutos. Concentrar el sobrenadante obtenido a 30 mililitros a fuego medio.Transfiera el concentrado final a un plato y séquelo con un horno de secado eléctrico hasta que solo quede el soluto en forma de polvo. Ahora, pesa el polvo obtenido y disuélvelo en agua destilada estéril para crear la dosis diaria para ratones. Para preparar ácido cinco aminoácidos salicílico, pese 64 miligramos de polvo de ácido cinco aminoácidos salicílico y disuélvalo en 200 mililitros de agua destilada estéril.

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Research

JoVE Journal

Medicine

Preparation of Liujunzi Decoction (LJZD) for the Treatment of Ulcerative Coloproctitis-Colorectal Cancer  (UC-CRC)

52 vistas. Para comenzar, coloque a Dang Shen, Bai Zhu, en una olla de cerámica especializada. Agrega 1000 mililitros de agua destilada y déjala en remojo a temperatura ambiente durante una hora. Con un molinillo, muele 12 gramos de Fu ling hasta obtener un polvo fino.Luego, remoje el Fu ling en polvo en 300 mililitros de agua destilada en un recipiente separado durante una hora a temperatura ambiente. Mezcle la mezcla de hierbas chinas preparada en la olla de cerámica especializada. Hervir la...

Video: Preparación de la decocción de Liujunzi (LJZD) para el tratamiento de la coloproctitis ulcerosa-cáncer colorrectal (UC-CRC)

Establishment of Coloproctitis Cancer Model in Mice and Evaluation of Therapeutic Effect of Chinese Medicine

Colorectal cancer (CRC) is a common malignancy of the digestive system and has become the third most common malignancy worldwide and the second leading cause of malignancy-related death. Ulcerative coloproctitis (UC) is a precancerous lesion, and UC-associated CRC (UC-CRC) is the most common subtype of CRC. Therefore, a reasonable UC-CRC model is the cornerstone and guarantee of new drug development. Traditional Chinese medicine (TCM) has been widely used in the treatment of UC-CRC due to its good efficacy. As a classic tonic prescription of TCM, Liujunzi decoction (LJZD) has been widely used in the treatment of UC-CRC. In this study, a UC-CRC model was established by combining azomethane and dextran sulfate sodium, and the LJZD was administered. The data confirmed that LJZD can effectively inhibit cancer transition in UC-CRC by using mouse body weight, colorectal length, pathological and inflammatory factors, colorectal barrier function, and cancer markers. This protocol provides a system for evaluating the efficacy of TCM in the prevention and treatment of UC-CRC.

This protocol provides a mouse model of ulcerative coloproctitis-associated colorectal cancer induced by azomethane combined with dextran sulfate sodium. The model was used to evaluate the efficacy of traditional Chinese medicine compounds in the prevention and treatment of colorectal cancer.

Colorectal cancer (CRC) is a common malignancy of the digestive system and has become the third most common malignancy worldwide and the second leading ...