SPR Analysis to Determine the Binding Affinities of Vitamin A Membrane Receptors with Their Physiological Ligand RBP4

Begin by immobilizing the purified msRBP4 protein on the sensor chip. To do so, go to the Run menu, choose the wizard, and proceed with the immobilization option. Next, dilute the ligand in immobilization buffer to a concentration of 10 micrograms