eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Conjugation of IR700 with mAb
<ol>
	<li>Incubate monoclonal antibody (mAb) (1 mg, 6.8 nmol) with IR700 N-Hydroxysuccinimide (NHS) ester (66.8 mg, 34.2 nmol, 5 mmol/L in dimethyl sulfoxide [DMSO]) in 0.1 mol/L Na2HPO4 (pH 8.6) at 15-25 &#176;C for 1 h.</li>
	<li>Purify the mixture using a column (e.g., Sephadex). Prepare and wash the column with phosphate-buffered saline (PBS). Then, apply the mixture onto the column and collect the drop, which contains the purified IR700-conjugated antibody. This IR700-conjugated antibody is called the antibody photosensitizer conjugate (APC).</li>
	<li>Measure the protein and IR700 concentration in the APC.
	<ol>
		<li>Prepare calibration curves for protein and IR700 using a spectrophotometer.</li>
		<li>Mix standard albumin concentrations with a protein assay kit following the kit protocol (see Table of Materials, Coomassie Brilliant Blue (CBB) protein staining). Measure the absorbance of albumin at 595 nm wavelength, and plot the protein&#39;s calibration curve (a linear approximation formula) using the following equation: y = ax + b (x: concentration, y: absorbance).</li>
		<li>Obtain calibration curves for IR700 with absorption at 690 nm using the same procedure. The standard concentration of IR700 is recommended at 0.1-5 &#181;M (0.1954-9.77 &#181;g/mL).</li>
		<li>Measure the protein concentration and IR700 concentration in the APC using a calibration curve [x = (y-b)/a (x: concentration, y: absorbance)].</li>
		<li>Determine the number of IR700 dyes bound per mAb with the results of the molar concentration. 
		NOTE: Determining the optimal conjugation number of IR700 molecules per mAb molecule is important. Generally, approximately three IR700 molecules bound on a single mAb molecule would be effective both in vitro and in vivo. Many IR700 bound per antibody (e.g., six) makes it easier to be trapped in the liver during in vivo experiments. The ratio of antibody bound to IR700 was in the range of 1:2-1:4. The proportion of IR700 was reduced, if necessary.</li>
	</ol>
	</li>
	<li>Perform sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as a confirmation for the formation of an APC. Image the gel at 700 nm using a fluorescent imager, and stain the protein in the gel using a protein staining kit following the kit protocol (see Table of Materials, CBB protein staining).</li>
</ol>
2. Generation of a pleural dissemination model
<ol>
	<li>Prepare luciferase-expressing target cells and suspend 1.0 &#215; 106 target cells in 100 &#181;L of PBS. 
	NOTE: Intrathoracic cancer cells, such as lung cancer and MPM, are suitable target cells. Luciferase-expressing cells were prepared via luciferase gene transfection, and high expression of luciferase was confirmed after &#62; 10 cell passages. Cells were cultured in a medium supplemented with 10% fetal bovine serum, penicillin (100 IU/mL), and streptomycin (100 mg/mL). The number of cells was adjusted according to the tumor growth rate and the time course of treatment (1.0. &#215; 105-6.0 &#215; 106 cells/body weight).</li>
	<li>Prepare 8-12-week-old female homozygote athymic nude mice with a preferable body weight of 19-21 g.</li>
	<li>Anesthetize mice during the procedure with isoflurane (introduction: 4-5%, maintenance: 2-3%); press the tail with tweezers to confirm no reaction.</li>
	<li>Make a stopper with polystyrene foam and attach the stopper to the 30 G needle so that the tip remains at 5 mm to prevent lung injury. Bend the needle tip with clean forceps or press it against a hard object cleaned with 70% EtOH to avoid pneumothorax (Figure 1). 
	CAUTION: Be careful not to pierce oneself. Use forceps to bend the needle. Do not hold the stopper when attaching it to the needle. Sticking the stopper before filling the cells into the syringe is safer.</li>
	<li>Fill a syringe (1 mL) with target cells and attach a 30G needle with a stopper.</li>
	<li>Pierce a needle into the mouse&#39;s chest through the intercostal space. Owing to the resistance while hitting against the ribs at that time, the needle tip moved up and down. After passing through the intercostal space, press the syringe against the mouse and inject 100 &#181;L of target cells (Figure 2). 
	NOTE: The mouse breaths deeply when the needle correctly enters the chest cavity. With the bending of the needle tip, pneumothorax and inappropriate injection of cells into the lung could be avoided. 
	NOTE: Right chest wall puncture is recommended to avoid the risk of cardiac wall puncture.</li>
	<li>Roll the mouse 2-3 times to spread the cells throughout the thoracic cavity.</li>
	<li>Return the mouse to a clean and warm cage and monitor until ambulatory. After the procedure, the mouse will wake up from anesthesia and behave normally.</li>
</ol>
3. Measurement of bioluminescence
NOTE: The software used for data acquisition is listed in the Table of Materials.
<ol>
	<li>To confirm the generation of the pleural dissemination model, evaluate the bioluminescence images daily after injecting the cells into the thoracic cavity.</li>
	<li>Anesthetize mice (step 2.3), and inject intraperitoneally with D-luciferin (15 mg/mL, 200 &#181;L).</li>
	<li>Ten minutes after the injection, set the mouse in the bioluminescence imaging (BLI) measuring equipment. For image acquisition, open the software&#39;s Acquisition Control Panel. Select Luminescent, Photograph, and Overlay (Figure 3).</li>
	<li>Set exposure time as Auto. Set Binning as small.</li>
	<li>Set f/stop as 1 for luminescent and 8 for photograph; f/stop controls the amount of light the charged-coupled device detector receives.</li>
	<li>Set the Field of View as C.</li>
	<li>Once the mouse sample is ready for imaging, click Acquire for imaging acquisition. Mice with sufficient luciferase activity were selected for further studies. 
	NOTE: A suitable pleural dissemination model shows strong luminescence on the diffused site in the chest when viewed from the ventral side. If the BLI images are not diffused in the thorax and only at the injection site, the tumor may be transplanted subcutaneously.</li>
	<li>After displaying the image, set the display format to Radiance. Open the Tool Palette panel (Figure 4A).</li>
	<li>Select ROI Tools. We recommend using the Circle to range the bioluminescent area on images.</li>
	<li>Click Measure ROIs to measure the surface bioluminescent intensity (Figure 4B).</li>
	<li>Use Configure Measurement on the left corner of the ROI measurement panel to select the values/information needed. Export this data table as a .csv file (Figure 4C).</li>
	<li>Use the values of Total Flux (p/s) as the bioluminescent intensity quantification in the .csv file.</li>
</ol>
4. Diffuse luminescence imaging tomography (DLIT)
NOTE: The software used for data acquisition is listed in the Table of Materials.
<ol>
	<li>Turn on the X-ray Armed button.</li>
	<li>Anesthetize the mice (step 2.3) and inject D-luciferin (15 mg/mL, 200 &#181;L) intraperitoneally into the mice. To shoot DLIT continuously from 3.2 to 3.7, skip this step.</li>
	<li>Ten minutes after injection, set the mouse in the BLI equipment.</li>
	<li>Open the Acquisition Control Panel of the software. Select Luminescent, Photograph, CT, Standard-One Mouse, and Overlay. Other settings were the same as in 3.4-3.6 (Figure 5A).</li>
	<li>Select the Imaging Wizard on the Acquisition Control Panel.</li>
	<li>Select Bioluminescence and then DLIT (Figure 5B).</li>
	<li>Select Firefly as the wavelength to measure (Figure 5C).</li>
	<li>Set the Imaging Subject as Mouse, Exposure parameters as Auto Settings, Field of View as C-13.4 cm, and Subject Height as 1.5 cm (Figure 5D).</li>
	<li>Push the X-rays, which will be produced when energized. Acquire.</li>
	<li>Open the computed tomography (CT) sequential image data.</li>
	<li>Open Surface Topography on the Tool Palette. Select Show (Figure 6A).</li>
	<li>Adjust the threshold, as the purple display shows only the body surface (Figure 6B). Then, select the Subject Nude Mouse and click the Generate Surface. Ensure that the mouse&#39;s outline is accurately drawn (Figure 6C).</li>
	<li>Open the Tool Palette, DLIT 3D Reconstruction Properties tab. Select Tissue Properties as Mouse Tissue and Source Spectrum as Firefly (Figure 6D). Next, open the Analyze tab and confirm the data for each selected wavelength data. Finally, click the Reconstruct button (Figure 6E).</li>
	<li>Confirm the presence of BLI in the chest cavity in the configured DLIT image.</li>
</ol>
5. NIR-PIT for in vivo pleural dissemination model
<ol>
	<li>Measure the light dose of a 690 nm wavelength (NIR) laser with a power meter and adjust the output to 100 mW/cm2. 
	NOTE: The laser light is coherent with a precise coil size. As a result, the light energy hardly changes regardless of the distance within 50 cm. If there are many adverse events, such as burns, reduce the output within the 40 mW/cm2 range.</li>
	<li>Clean the tail with 70% EtOH and inject APC (100 &#181;g) intravenously via the tail vein 24 h before NIR irradiation. Apply pressure to the injection site to control bleeding. 
	NOTE: Adjust the volume of APC to 50-200 &#181;L for injection.</li>
	<li>Anesthetize the mice (step 2.3) and lay them on their back. To avoid NIR irradiation to the non-target site, shield other sites with aluminum foil (Figure 7A). Irradiate with NIR light with a laser of 100 J/cm2; if the tumor is disseminated back to the belly, the NIR-light irradiation dose could be divided in multiple directions (Figure 7B). 
	NOTE: Adjust the dose at 30-150 J/cm2 depending on the in vitro results and adverse events such as burns.</li>
	<li>When the NIR irradiation is complete, and the mouse is awake, return it to the cage.</li>
	<li>Observe the BLI and measure the ROI over time (every day) (See 3.2-3.12).</li>
	<li>For ex vivo imaging, euthanize mice with carbon dioxide 24 h after the APC injection, immediately before the NIR irradiation.
	<ol>
		<li>To observe the inside of the mouse&#39;s chest, remove the thorax and cut the ribs and sternum. Capture the fluorescence image (700 nm) alongside the control without APC administration. Then, D-luciferin (150 &#181;g/mL) was applied over the exposed thorax, and the BLI was taken (refer 3.2-3.7).</li>
	</ol>
	</li>
</ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>0.25w/v% Trypsin-1mmol/l EDTA 4Na Solution with Phenol Red</td>
			<td>Wako</td>
			<td>209-016941</td>
			<td>for cell culture</td>
		</tr>
		<tr>
			<td>1mL syringe</td>
			<td>TERUMO</td>
			<td>SS-01T</td>
			<td>for mice experiment</td>
		</tr>
		<tr>
			<td>30G needle</td>
			<td>Nipro</td>
			<td>1907613</td>
			<td>for mice experiment</td>
		</tr>
		<tr>
			<td>BALB/cSlc-nu/nu</td>
			<td>Japan SLC</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Collidal Blue Staining Kit</td>
			<td>Invitrogen</td>
			<td>LC6025</td>
			<td>use for gel protein staining</td>
		</tr>
		<tr>
			<td>Coomassie (bradford) Plus protein assay</td>
			<td>Thermo Fisher Scientific Inc (Waltham, MA, USA)</td>
			<td>PI-23200</td>
			<td>for measuring the APC concentration</td>
		</tr>
		<tr>
			<td>Dimethyl sulfoxide (DMSO)</td>
			<td>Wako</td>
			<td>043-07216</td>
			<td>use for conjugation of IR700</td>
		</tr>
		<tr>
			<td>D-Luciferin (potassium salt)</td>
			<td>Cayman Chemical</td>
			<td>14681</td>
			<td>for bioluminescence imaging and DLIT</td>
		</tr>
		<tr>
			<td>GraphPad Prism7</td>
			<td>GraphPad software</td>
			<td></td>
			<td>for statistical analysis</td>
		</tr>
		<tr>
			<td>Image Studio</td>
			<td>Li-Cor Biosciences</td>
			<td></td>
			<td>for analyzing 700 nm fluorescent image</td>
		</tr>
		<tr>
			<td>IRDye 700DX Ester Infrared Dye</td>
			<td>LI-COR Bioscience (Lincoln, NE, USA)</td>
			<td>929-70011</td>
			<td></td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>Wako</td>
			<td>095-06573</td>
			<td>for mice anesthesia</td>
		</tr>
		<tr>
			<td>IVIS Spectrum CT</td>
			<td>PerkinElmer</td>
			<td></td>
			<td>for capturing bioluminescent image and DLIT</td>
		</tr>
		<tr>
			<td>Living Image</td>
			<td>PerkinElmer</td>
			<td></td>
			<td>for analyzing bioluminescent image and DLIT</td>
		</tr>
		<tr>
			<td>Na2HPO4</td>
			<td>SIGMA-ALDRICH (St. Louis, MO, USA)</td>
			<td>S9763</td>
			<td>use for conjugation of IR700</td>
		</tr>
		<tr>
			<td>NIR Laser</td>
			<td>Changchun New Industries Optoelectronics Technology</td>
			<td>MRL-III-690R</td>
			<td>for NIR irradiation</td>
		</tr>
		<tr>
			<td>Novex WedgeWell 4 to 20%, Tris-Glycine, 1.0 mm, Mini Protein Gel, 12 well</td>
			<td>Invitrogen</td>
			<td>XP04202BOX</td>
			<td>use for SDS-PAGE</td>
		</tr>
		<tr>
			<td>NuPAGE LDS Sample Buffer (x4)</td>
			<td>Invitrogen</td>
			<td>NP0007</td>
			<td>use for SDS-PAGE</td>
		</tr>
		<tr>
			<td>Optical power meter</td>
			<td>Thorlabs (Newton, NJ, USA)</td>
			<td>PM100</td>
			<td>for measuring the output of the NIR laser</td>
		</tr>
		<tr>
			<td>PBS(-)</td>
			<td>Wako</td>
			<td>166-23555</td>
			<td></td>
		</tr>
		<tr>
			<td>Pearl Trilogy imaging system</td>
			<td>Li-Cor Biosciences</td>
			<td></td>
			<td>for capturing 700 nm fluorecent image</td>
		</tr>
		<tr>
			<td>Penicilin-Streptomycin Solution (x100)</td>
			<td>Wako</td>
			<td>168-23191</td>
			<td>for cell culture</td>
		</tr>
		<tr>
			<td>Puromycin Dihydrochloride</td>
			<td>ThermoFisher</td>
			<td>A1113803</td>
			<td>for luciferase transfection</td>
		</tr>
		<tr>
			<td>RediFect Red-Fluc-Puromycin Lentiviral Prticles</td>
			<td>PerkinElmer</td>
			<td>CLS960002</td>
			<td>for luciferase transfection</td>
		</tr>
		<tr>
			<td>RPMI-1640 with L-glutamine and Phenol Red</td>
			<td>Wako</td>
			<td>189-02025</td>
			<td>for cell culture</td>
		</tr>
		<tr>
			<td>Sephadex G25 column (PD-10)</td>
			<td>GE Healthcare (Piscataway, NJ, USA)</td>
			<td>17-0851-01</td>
			<td>use for conjugation of IR700</td>
		</tr>
		<tr>
			<td>UV-1900i</td>
			<td>Shimadzu</td>
			<td></td>
			<td>for measuring the APC concentration</td>
		</tr>
	</tbody>
</table>

a near-infrared photoimmunotherapy technique to treat lung cancer in a murine model

Source: Yasui, H., et al. <a href="https://app.jove.com/v/61593">Near Infrared Photoimmunotherapy for Mouse Models of Pleural Dissemination.</a> J. Vis. Exp. (2021)
This video demonstrates a near-infrared photoimmunotherapy technique to treat lung cancer in an engineered mouse model exhibiting luciferase-expressing tumor cells. Upon injecting an antibody-photosensitizer conjugate (APC) that binds to specific antigens on the tumor cells, the mouse is exposed to near-infrared radiation, inducing photochemical changes in the APC and leading to necrotic death of the bound tumor cells. The effectiveness of the treatment is monitored by injecting luciferin, a luciferase substrate, and measuring the bioluminescence over time, correlating with tumor growth.

<img alt="Figure 1" src="/files/ftp_upload/22225/22225_Figure1.jpg" /> 
Figure 1: Easy hand-made device for cell transplantation. Attach the stopper made with polystyrene foam to the 30G needle so that the tip remains at 5 mm. The tip of the needle should be bent to avoid pneumothorax.
<img alt="Figure 2" src="/files/ftp_upload/22225/22225_Figure2.jpg" /> 
Figure 2: Injection of target cells in...

A Near-Infrared Photoimmunotherapy Technique to Treat Lung Cancer in a Murine Model

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

Take an immunodeficient mouse that exhibits tumor growth in the pleura, a double-layered membrane lining the chest cavity.
The engineered tumor cells express the enzyme luciferase.
Intravenously inject an antibody-photosensitizer conjugate, or APC, comprising a tumor antigen-specific antibody bound to a photoabsorber dye.
The control tumor-bearing mouse does not receive the injection.
In the treated mouse, APCs reach the pleural cavity and bind to tumor cell-surface antigens.
Anesthetize the mouse and expose the chest to near-infrared radiation.
Radiation-induced dye photolysis changes the APC shape, inducing stress and increasing transmembrane water flow, resulting in tumor cell bursting and necrotic death.
In the control mouse, tumor cells remain unaffected.
Intraperitoneally inject the bioluminescent substrate luciferin. Upon entering viable tumor cells, luciferin gets oxidized by luciferase, emitting light.
Measure the bioluminescence at predetermined intervals.
A decreased bioluminescence in the treated mouse compared to the increased bioluminescence in the control mouse from tumor growth confirms photoimmunotherapy-mediated tumor destruction.

a-near-infrared-photoimmunotherapy-technique-to-treat-lung-cancer

Research

JoVE Encyclopedia of Experiments

Immunology

Immunotherapy

Model Development and Disease Study

89 vistas. Fuente: Yasui, H., et al. Fotoinmunoterapia de infrarrojo cercano para modelos de ratón de diseminación pleural. J. Vis. Exp. (2021) Este video muestra una técnica de fotoinmunoterapia de infrarrojo cercano para tratar el cáncer de pulmón en un modelo de ratón diseñado que exhibe células tumorales que expresan luciferasa. Al inyectar un conjugado anticuerpo-fotosensibilizador (APC) que se une a antígenos específicos en las células tumorales, el ratón se expone a la radiación infrar...

Video: Una técnica de fotoinmunoterapia de infrarrojo cercano para tratar el cáncer de pulmón en un modelo murino - Concepto

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Cancer-associated fibroblasts (CAFs) are key actors in the context of the tumor microenvironment. Despite being reduced in number as compared to tumor cells, CAFs regulate tumor progression and provide protection from antitumor immunity. Emerging anticancer strategies aim to remodel the tumor microenvironment through the ablation of pro-tumorigenic CAFs or reprogramming of CAFs functions and their activation status. A promising approach is the development of nanosized delivery agents able to target CAFs, thus allowing the specific delivery of drugs and active molecules. In this context, a cellular model of CAFs may provide a useful tool for in vitro screening and preliminary investigation of such nanoformulations.
This study describes the isolation and culture of primary CAFs from the syngeneic 4T1 murine model of triple-negative breast cancer. Magnetic beads were used in a 2-step separation process to extract CAFs from dissociated tumors. Immunophenotyping control was performed using flow cytometry after each passage to verify the process yield. Isolated CAFs can be employed to study the targeting capability of different nanoformulations designed to tackle the tumor microenvironment. Fluorescently labeled H-ferritin nanocages were used as candidate nanoparticles to set up the method. Nanoparticles, either bare or conjugated with a targeting ligand, were analyzed for their binding to CAFs. The results suggest that ex vivo extraction of breast CAFs may be a useful system to test and validate nanoparticles for the specific targeting of tumorigenic CAFs.

This paper aims to provide a protocol for the isolation and culture of primary cancer-associated fibroblasts from a syngeneic murine model of triple-negative breast cancer and their application for the preclinical study of novel nanoparticles designed to target the tumor microenvironment.&#160;

Aislamiento de fibroblastos asociados al cáncer primario a partir de un modelo murino singénico de cáncer de mama para el estudio de nanopartículas dirigidas

Cancer-associated fibroblasts (CAFs) are key actors in the context of the tumor microenvironment. Despite being reduced in number as compared to tumor ...

Investigación

JoVE Journal

Investigación sobre el cáncer

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy

The precision delivery of anti-cancer agents which aim for targeted and deep-penetrated delivery as well as a controlled release at the tumor site has been challenged. Here, we fabricate iron oxide nanoparticle shelled microbubbles (NSMs) through self-assembly, synergizing magnetic, acoustic, and optical responsiveness in one nanotherapeutic platform. Iron oxide nanoparticles serve as both magnetic and photothermal agents. Once intravenously injected, NSMs can be magnetically guided to the tumor site. Ultrasound triggers the release of iron oxide nanoparticles, facilitating the penetration of nanoparticles deep into the tumor due to the cavitation effect of microbubbles. Thereafter, magnetic hyperthermia and photothermal therapy can be performed on the tumor for combinational cancer therapy, a solution for cancer resistance due to the tumor heterogeneity. In this protocol, the synthesis and characterization of NSMs including structural, chemical, magnetic and acoustic properties were performed. In addition, the anti-cancer efficacy by thermal therapy was investigated using in vitro cell cultures. The proposed delivery strategy and combination therapy holds great promise in cancer treatment to improve both delivery and anticancer efficacies.

Presented here is a protocol for the fabrication of iron oxide nanoparticle-shelled microbubbles (NSMs) through self-assembly, synergizing magnetic, acoustic, and optical responsiveness in one nanotherapeutic platform for magnetic hyperthermia and photothermal combination cancer therapy.

Microburbujas magnéticas, acústicas y ópticas de triple respuesta para la hipertermia magnética y la terapia combinada de cáncer potototérmico

The precision delivery of anti-cancer agents which aim for targeted and deep-penetrated delivery as well as a controlled release at the tumor site has ...

Bioingeniería

Left Lung Orthotopic Transplantation in a Juvenile Porcine Model for ESLP

Lung transplantation is the gold-standard treatment for end-stage lung disease, with over 4,600 lung transplantations performed worldwide annually. However, lung transplantation is limited by a shortage of available donor organs. As such, there is high waitlist mortality. Ex situ lung perfusion (ESLP) has increased donor lung utilization rates in some centers by 15%-20%. ESLP has been applied as a method to assess and recondition marginal donor lungs and has demonstrated acceptable short- and long-term outcomes following transplantation of extended criteria donor (ECD) lungs. Large animal (in vivo) transplantation models are required to validate ongoing in vitro research findings. Anatomic and physiologic differences between humans and pigs pose significant technical and anesthetic challenges. An easily reproducible transplant model would permit the&#160;in vivo&#160;validation of current ESLP strategies and the preclinical evaluation of various interventions designed to improve donor lung function. This protocol describes a porcine model of orthotopic left lung allotransplantation. This includes anesthetic and surgical techniques, a customized surgical checklist, troubleshooting, modifications, and the benefits and limitations of the approach.

This protocol describes a juvenile porcine model of orthotopic left lung allotransplantation designed for use with ESLP research. Focus is made on anesthetic and surgical techniques, as well as critical steps and troubleshooting.

Trasplante ortotópico de pulmón izquierdo en un modelo porcino juvenil para LESP

Lung transplantation is the gold-standard treatment for end-stage lung disease, with over 4,600 lung transplantations performed worldwide annually. ...

A Near-Infrared Photoimmunotherapy Technique to Treat Lung Cancer in a Murine Model

Transcripción

A Near-Infrared Photoimmunotherapy Technique to Treat Lung Cancer in a Murine Model