Monitoring the Brain Cortex with a Multi-Electrode Array in Response to Seizure-Inducing Agents

Start with a brain slice containing the anterior cingulate cortex, or ACC, and the thalamus regions.

Using an anchor, secure the brain slice to a multi-electrode array or MEA chamber, an electric signal-measuring device perfused with oxygenated artificial cerebrospinal fluid.

Position transcranial direct-current stimulation or tDCS electrodes with one near the ACC and the other placed distal to the ACC to deliver direct current to the brain slice.

Record baseline signals and deliver electric current to the slice through tDCS.

Adjust the electrode length and the tDCS current to obtain quality signals.

Using a stimulating electrode, deliver pulses of current to the thalamus, which induces artificial seizure by stimulating the ACC.

Adjust the current to stimulate the thalamus-cingulate pathway, which connects the thalamus to the ACC, to obtain an optimal ACC response.

Record it through the MEA.

Infuse seizure-inducing drugs into the MEA chamber

Using stimulating electrodes, deliver electric pulses to the thalamus-cingulate pathway and record the drug-induced ACC response.