Embryonic Brain Preparation and Vibratome Sectioning
5:09
Slice Culture and Time-lapse Imaging
6:21
Results: Dopaminergic Neuronal Development in Organotypic Slice Cultures of Embryonic Ventral Midbrain
7:04
Conclusion
A method to generate organotypic slices from the E12.5 murine embryonic midbrain is described. The organotypic slice cultures can be used to observe the behavior of dopaminergic neurons or other ventral midbrain neurons.