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Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

13.3K Views

11:33 min

March 12th, 2013

March 12th, 2013

13,259 Views

0:05

Title

1:37

Animal Anesthesia and Organ Harvesting

3:00

Preparation of Heart and Langendorff Perfusion

4:03

Dissociation of Cardiomyocytes

6:30

Preparation of Cardiomyocytes for Cellular Electrophysiology, Biochemical of IF Studies

8:53

Cellular Electrophysiology

10:33

Results: Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

11:04

Conclusion

Kv channel dysfunction is associated with cardiac arrhythmias. In order to study the molecular mechanisms that lead to such arrhythmias we utilize a systematic protocol for isolation of atrial and ventricular cardiomyocytes from Kv channel ancillary subunit knockout mice. Isolated cardiomyocytes can then immediately be used for cellular electrophysiological studies, biochemical or immunofluorescence (IF) assays.

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