Cell Labeling (Standard Protocol with Coincubation)
2:57
Determination of 19F/cell using 19F NMR
4:17
Imaging and Mouse Preparation
5:29
B1 Correction
7:44
Image Processing
8:45
Results: 19F-labeled Cells Homing to a Draining Lymph Node
9:35
Conclusion
We describe a general protocol for in vivo cell tracking using MRI in a mouse model with ex vivo labeled cells. A typical protocol for cell labeling, image acquisition processing and quantification is included.