Prepare Samples for Tandem Affinity Purification Procedure
4:02
Tandem Affinity Purification Procedure
7:44
Results: Identification of Interacting Proteins in the Drosophila Brain by Tandem Affinity Purification
9:31
Conclusion
Drosophila is famous for its powerful genetic manipulation, but not for its suitability of in-depth biochemical analysis. Here we present a TAP-based procedure to identify interacting partners of any protein of interest from the fly brain. This procedure can potentially lead to new avenues of research.