May 10th, 2014
•This paper provides a step by step guide to the fluctuation analysis technique k-Space Image Correlation Spectroscopy (kICS) for measuring diffusion coefficients of fluorescently labeled plasma membrane proteins in live mammalian cells.
Videos relacionados
Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
Crystallization of Membrane Proteins in Lipidic Mesophases
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
Imaging Plasma Membrane Deformations With pTIRFM
Using Digital Image Correlation to Characterize Local Strains on Vascular Tissue Specimens
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados