Identification of Cy3-positive Cells via Flow Cytometry
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Results: Detection of Pluripotency Marker Gene Expression in Live iPSCs and Embryonic Stem Cells
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Conclusion
This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.