GFP-expressing Retrovirus Glioblastoma (GBM) Cell Labeling and Time-lapse Single Photon Laser Scanning Confocal Imaging
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Imaging Post-processing and Tumor Cell Tracking
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Results: Representative Human GBM Imaging and Analyses
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Conclusion
Transcribir
The overall goal of this procedure is to observe and analyze invasive migratory behaviors in primary human glioblastoma cells. This method can help answer key questions in the field of neurooncology, such as what are the phenotypic and molecular p
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Current ex vivo models of glioblastoma (GBM) are not optimized for physiologically relevant study of human tumor invasion. Here, we present a protocol for generation and maintenance of organotypic slice cultures from fresh human GBM tissue. A description of time-lapse microscopy and quantitative cell migration analysis techniques is provided.