Results: Frozen GI Tissue Primary MF Culture Generation
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Conclusion
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The overall goal of this method is to isolate viable mucosal myofibroblasts and fibroblasts from frozen gastrointestinal tissue samples, originally obtained from biopsies or surgical specimens. The isolation of myofibroblasts and fibroblasts from
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Here, a protocol to isolate and establish primary fibroblast/myofibroblast (MF) cultures from frozen gastric, small intestinal, and colonic tissue-yielding cells with a MF phenotype-is presented. These cells express CD90, α-SMA and vimentin. MFs can be used for a variety of functional assays including enzymatic activity and cytokine production.