The overall goal of this method is to objectively quantitate protein expression and co-localization using multispectral imaging. This method can help answer key questions in the basic research and diagnostic pathology field, such as how proteins c
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Immunohistochemistry is a powerful lab technique for evaluating protein localization and expression within tissues. Current semi-automated methods for quantitation introduce subjectivity and often create irreproducible results. Herein, we describe methods for multiplexed immunohistochemistry and objective quantitation of protein expression and co-localization using multispectral imaging.