Exogenously Formed Clot Preparation and Fluorescent Marker Labeling
2:55
Thromboembolic Stroke Model Preparation
5:42
In Vivo MicroCT Cerebral Thrombus Imaging
6:36
Ex Vivo Near Infrared Fluorescent (NIRF) Thrombus Imaging and MicroCT Thrombus Area Imaging Quantification
9:48
Results: Representative Direct Cerebral Thrombus Imaging before and after Thrombolysis Activation
11:28
Conclusion
Transcribir
The overall goal of this direct thrombus imaging technique is to quantitatively visualize fluorescently labeled cerebral thromboemboli in vivo and ex vivo, using gold nanoparticles, computed tomography, and near-infrared fluorescent imaging. This
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This protocol describes the application of combined near-infrared fluorescent (NIRF) imaging and micro-computed tomography (microCT) for visualizing cerebral thromboemboli. This technique allows the quantification of thrombus burden and evolution. The NIRF imaging technique visualizes fluorescently labeled thrombus in excised brain, while the microCT technique visualizes thrombus inside living animals using gold-nanoparticles.