Evaluating MicroRNA-targeting Specificity: Virus-spreading Assay Using Synthetic MicroRNA Mimics
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Results: Comparing MicroRNA-targeted Virus to Unmodified Virus
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Conclusion
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The overall goal of the following experiments is to assess the efficiency and specificity of microRNA-based regulation of picornavirus tropism in vitro. This is achieved by preparing picornavirus encoding microRNA response elements in their genome
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We describe here a method for regulating picornavirus tropism by incorporating sequences complementary to specific microRNAs into the viral genome. This protocol can be adapted to all different classes of viruses with modifications based upon the length and nature of their life cycle.