Setting Up the Microscope and Acquisition Protocol
3:10
Loading of the Cells with CM-H2DCFDA and TMRM
4:18
First and Second Live Imaging Rounds
6:22
Image Processing and Analysis
9:35
Results: Effect of Saquinavir on Primary Human Fibroblasts
10:38
Conclusion
Transcribir
The overall goal of this high-content microscopy-based method is to simultaneously quantify mitochondrial morphofunction and cellular levels of reactive oxygen species so as to establish a robust redox fingerprint for cell lines exposed to differe
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This paper presents a high-content microscopy workflow for simultaneous quantification of intracellular ROS levels, as well as mitochondrial membrane potential and morphology – jointly referred to as mitochondrial morphofunction – in living adherent cells using the cell-permeant fluorescent reporter molecules 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) and tetramethylrhodamine methylester (TMRM).