August 23rd, 2017
•Here, a method is described for the preparation of 3-dimensional (3D) spheroid co-culture of pancreatic cancer cells and fibroblasts, followed by measurement of metabolic functions using an extracellular flux analyzer.
Videos relacionados
Assessment of the Metabolic Profile of Primary Leukemia Cells
A Spheroid Killing Assay by CAR T Cells
Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
Mimicking and Manipulating Pancreatic Acinar-to-Ductal Metaplasia in 3-dimensional Cell Culture
Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures
Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados