Melanoma Cell-derived Factor Harvest and Bone Marrow-derived Adipocyte Induction
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Red Oil O Staining and Quantitative Polymerase Chain Reaction (qPCR) Analysis
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Bone Marrow Adipocyte-melanoma Cell Co-culture
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Results: Dynamic Bone Marrow-derived Adipocyte Regulation of Tumor Cells
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Conclusion
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This method can help answer key questions in the bone metastasis research field, such as what changes are induced in the bone marrow adipocyte growth and their development in a cancer microenvironment. The big advantages of this technique are that
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Here, we present a reliable and straightforward two-dimensional (2D) coculture system for studying the interaction between tumor cells and bone marrow adipocytes, which reveals a dual effect of melanoma cell-derived factors on the bone marrow adipocytes differentiation and also poses a classic method for the mechanistic study of bone metastasis.