Exposure, Identification, and Fixation of the Vertebrae at the Lumbar Spinal Cord Level
4:08
Preparation of the Injection Syringe
5:25
Intraspinal Injections
7:12
Results: Intraspinal Injection of AAV1-eGFP/AAV1-flex-eGFP
8:14
Conclusion
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The overall goal of intraspinal delivery of recombinant adeno-associated viruses is to enable the histological labeling and functional manipulation of genetically defined neurons in the dorsal spinal cord. This method can help answer key questions
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Intraspinal injection of recombinase dependent recombinant adeno-associated virus (rAAV) can be used to manipulate any genetically labelled cell type in the spinal cord. Here we describe how to transduce neurons in the dorsal horn of the lumbar spinal cord. This technique enables functional interrogation of the manipulated neuron subtype.