Visualization of Neutrophil Activation with Reverse-Inverso-MUB40-Cy5 Peptide
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Results: RI-MUB40 Staining Increases Over Time as More Neutrophils Become Activated
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Conclusion
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MUB40 is a novel neutrophil marker, which binds to lactoferrin and allows a specific detection in human and all mammal tissue sample tested so far. Labeling neutrophils with MUB40 is easy, quick, and specific. It's now used widely in our lab and t
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Here, we present a protocol to detect the presence of neutrophils in fixed/permeabilized histology sections and assess the activation state of live purified neutrophils. In particular, the MUB40 peptide binds lactoferrin present in neutrophil-specific and tertiary granules. Exposure of the granule contents through either permeabilization or neutrophil activation allows for the marking of neutrophils.